Translational_Unit

Part:BBa_K4382013:Design

Designed by: UZAIR MOTIWALA   Group: iGEM22_IISER_Bhopal   (2022-10-12)


Ligninase Gene Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1795
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 217
    Illegal BsaI site found at 226
    Illegal BsaI site found at 1192
    Illegal SapI site found at 622
    Illegal SapI site found at 980
    Illegal SapI.rc site found at 1136


Design Notes

The gene construct was codon optimized for Bacillus Subtilis.


Source

The gene construct was synthesized from IDT.

References